GLASS LAB · Medical Aesthetics Evidence Index 303 cells · 19,226 papers · 5,969 top-tier evidence

Wrinkle / Line · Glabellar lines

Botulinum toxin a evidence for Glabellar lines

A 69.5 / 100 Supported 117 RCT/meta · 37 clinical/observational · 17 case · 313 total
Strong evidence · positive conclusion

This procedure group has the highest level of PubMed evidence for this indication, and the papers broadly support its effectiveness.

Grade combines the quantity and quality of the research, while direction is a separate signal showing how the papers assess effectiveness.

Includes meta-analysis/SR Median year 2018 Last 5 years 37% Positive results 59% Negative results 16 Korea/Asia 31

Known in Korea as

톡신보톡스제오민디스포트코어톡스주름 보톡스다한증 보톡스디자인 보톡스사각턱 보톡스얼굴전체 톡신

Procedure and brand names vary, but the evidence above applies to the same procedure mechanism.

Key papers

tier1 Is Ready-To-Use AbobotulinumtoxinA More Effective Than OnabotulinumtoxinA for Glabellar Lines? A Randomized, Controlled, Triple-Blinded Clinical Trial. Journal of cosmetic dermatology · 2026 · PMID 41889091

Abstract summary (English) BACKGROUND: Most commercially available botulinum toxin A (BoNT-A) formulations are lyophilized or vacuum-dried powders requiring reconstitution. Recently a ready-to-use formulation (RTUaboBoNT-A) has been introduced. Although its efficacy in reducing glabellar lines has been reported, comparative data with powder onabotulinumtoxinA (OnaBoNT-A) remain limited.

Journal Article, Randomized Controlled Trial, Comparative Study

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tier1 NivobotulinumtoxinA in the Treatment of Lateral Canthal Lines Alone and With Concurrent Treatment of Glabellar Lines. Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] · 2025 · PMID 40923599

Abstract summary (English) BACKGROUND: Botulinum neurotoxins in aesthetic medicine require reconstitution, which may cause administration errors. OBJECTIVE: To evaluate liquid nivobotulinumtoxinA treatment of lateral canthal lines (LCL) and glabellar lines (GL). MATERIALS AND METHODS: Participants with moderate-to-severe LCL with/without moderate-to-severe GL were enrolled in 2 double-blind randomized clinical trials.

Journal Article, Randomized Controlled Trial

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tier1 Aesthetic Improvements Over Time: Long-Term Efficacy and Additional Outcomes of IncobotulinumtoxinA in the Simultaneous Treatment of Upper Facial Lines. Journal of cosmetic dermatology · 2025 · PMID 40968493

Abstract summary (English) BACKGROUND/OBJECTIVES: Two Phase III randomized controlled trials were conducted in the US (ULTRA I) and Germany (ULTRA II) to investigate the efficacy of incobotulinumtoxinA for simultaneous treatment of upper facial lines (UFLs: glabellar frown lines [GFLs], horizontal forehead lines [HFLs] and lateral canthal lines [LCLs]). PATIENTS/METHODS: Healthy adults with moderate to severe GFLs, HFLs, and symmetrical LCLs...

Journal Article, Randomized Controlled Trial, Clinical Trial, Phase III, Multicenter Study

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tier1 Patient-Reported Outcomes for Glabellar Line Improvement and Satisfaction With the RelabotulinumtoxinA Ready-to-Use Liquid Formulation: Data From the Phase 3 READY-1 Trial. Aesthetic surgery journal · 2025 · PMID 40242960

Abstract summary (English) BACKGROUND: RelabotulinumtoxinA (relaBoNT-A; Relfydess, Galderma, Uppsala, Sweden) is a novel, ready-to-use liquid form of botulinum toxin A, created using PEARL technology to produce a highly effective, complex-free formulation. OBJECTIVES: This study reports the patient-assessed effectiveness and satisfaction outcomes from the relaBoNT-A Phase 3 READY-1 study. METHODS: Adults with moderate-to-severe glabellar...

Journal Article, Randomized Controlled Trial, Clinical Trial, Phase III, Multicenter Study

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tier1 Efficacy and Safety of CKDB-501A in Treating Moderate-To-Severe Glabellar Lines: A Randomized, Double-Blind, Active-Controlled, Multi-Center Phase III Trial. Journal of cosmetic dermatology · 2025 · PMID 40586136

Abstract summary (English) BACKGROUND: Botulinum toxin is a key treatment for dynamic wrinkles. OBJECTIVE: This study evaluates CKDB-501A, a botulinum toxin completely free from animal-derived components including human-serum albumin, comparing its efficacy and safety to onabotulinumtoxinA (ONA) for the treatment of moderate-to-severe glabellar lines. METHODS: In this phase 3 trial, 300 subjects with moderate-to-severe glabellar lines were...

Journal Article, Clinical Trial, Phase III, Randomized Controlled Trial, Multicenter Study, Comparative Study

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tier1 Comparing Ready-to-Use and Powder AbobotulinumtoxinA for Glabellar Lines: A Randomized, Controlled, Triple-Blinded Clinical Trial. Journal of cosmetic dermatology · 2025 · PMID 40545952

Abstract summary (English) BACKGROUND: Botulinum toxin A (BoNT-A) is the most used procedure to treat glabellar lines; however, limited data exist about the effectiveness of ready-to-use BoNT-A (RTUaboBoNT-A) for this indication. AIMS: This study compared the efficacy, durability, and safety of RTUaboBoNT-A for moderate and severe glabellar wrinkles. METHODS: This randomized triple-blinded trial included 38 male volunteers aged between 25 and...

Journal Article, Randomized Controlled Trial, Comparative Study

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tier1 Efficacy and Safety of a Newly Developed Botulinum Toxin A (MBA-P01) in Patients with Moderate-to-Severe Glabellar Lines: A Randomized, Double-Blind, Active-Controlled, Multi-Center, Phase III Study with a Subgroup Analysis on Patients with COVID-19. Toxins · 2025 · PMID 40278658

Abstract summary (English) MBA-P01 is a newly developed botulinum toxin A (BoNT-A) product designed to provide similar clinical effects as OnabotulinumtoxinA (ONA-BoNT-A), thereby providing an alternative treatment option for glabellar lines. It is another holotoxin preparation containing BoNT-A1. This randomized, double-blind, active-controlled, multi-center, Phase III clinical trial aimed to evaluate the efficacy and safety of MBA-P01...

Journal Article, Clinical Trial, Phase III, Randomized Controlled Trial, Multicenter Study

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tier1 NivobotulinumtoxinA in the Treatment of Glabellar Lines With or Without Concurrent Treatment of Lateral Canthal Lines in Two Phase 3 Clinical Trials. Aesthetic surgery journal · 2025 · PMID 39587780

Abstract summary (English) BACKGROUND: Botulinum neurotoxins in aesthetic medicine require reconstitution before administration, which may be inconvenient and present errors among injectors. OBJECTIVES: The aim of this study was to evaluate the efficacy and safety of ready-to-use nivobotulinumtoxinA liquid formulation for the treatment of glabellar lines (GL) with or without treatment of lateral canthal lines (LCL). METHODS: Two multicenter,...

Journal Article, Clinical Trial, Phase III, Multicenter Study, Randomized Controlled Trial

View on PubMed
tier1 IncobotulinumtoxinA in the Treatment of Upper Facial Lines: Results From Two Randomized, Double-Blind, Placebo-Controlled, Phase III Studies. Aesthetic surgery journal · 2025 · PMID 39475143

Abstract summary (English) BACKGROUND: Two randomized, double-blind, placebo-controlled, Phase III studies of incobotulinumtoxinA for treating upper facial lines (UFLs; ie, a combination of glabellar frown lines [GFLs], horizontal forehead lines [HFLs], and lateral canthal lines [LCLs]) were conducted in the United States (ULTRA I: NCT04594213) and Germany (ULTRA II: NCT04622254). OBJECTIVES: The aim of this study was to evaluate the safety...

Journal Article, Randomized Controlled Trial, Clinical Trial, Phase III, Multicenter Study

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tier1 Meta-Analysis of Adverse Reactions of Botulinum Toxin A in Facial Rejuvenation Treatment. Aesthetic plastic surgery · 2024 · PMID 39623228

Abstract summary (English) BACKGROUND: Facial rejuvenation is a popular non-surgical treatment, and its treatment safety is of key significance to patients. The purpose of this meta-analysis is to compare the effects of botulinum toxin A and placebo on the occurrence of adverse reactions in facial rejuvenation treatment. OBJECTIVE: According to PRISMA guidelines, a comprehensive search was conducted in Chinese and English databases, and 20...

Journal Article, Meta-Analysis

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